Journal of Biomedical and Clinical Research 7(1): 25-30, doi: 10.1515/jbcr-2015-0121
Assessment of the Impact of Oxidative Stress on Frozen Seminal Plasma in Fertile and Infertile Men by Examining the Total Antioxidant Capacity
expand article infoVioleta S. Rilcheva, Nina P. Ayvazova, Danail I. Martinov, Cvetomir I. Ivanov, Emiliana I. Konova
© Violeta Rilcheva, Nina Ayvazova, Danail Martinov, Cvetomir Ivanov, Emiliana Konova.
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Citation: Rilcheva VS, Ayvazova NP, Martinov DI, Ivanov CI, Konova EI (2014) Assessment of the Impact of Oxidative Stress on Frozen Seminal Plasma in Fertile and Infertile Men by Examining the Total Antioxidant Capacity. Journal of Biomedical and Clinical Research 7(1): 25-30. https://doi.org/10.1515/jbcr-2015-0121
Open Access
Abstract
The aim of the study was to explore the impact of oxidative stress on frozen seminal plasma in fertile and infertile men by examining the total antioxidant capacity. Patients: Infertile patients from male infertility clinic with various diagnoses and fertile men. Design: Seminal plasma from proven fertile men [n=50] and infertile patients [n=50] were examined for total antioxidant capacity (TAC) level, semen parameters such as morphology, motility and concentration, and DNA integrity test. Interventions: Seminal plasma TAC measurement by luminometric assay using the TAC assay kit, semen analysis parameters, DNA integrity test. Fertile men showed higher TAC values (median and SD): 1201µM (SD±548), as compared with the infertile patients: 831μM (SD±343). The result from sperm morphology of fertile patients showed a mean percentage of 4.8 % (SD±1.68) whereas the percentage in the infertile group was 2.68% (SD ±1.68). The same group of samples, analyzed for DNA damage showed a mean of DFI 10.38% (SD±5.17%) in fertile men and a mean of DFI 17.22% (SD±7.22%) in infertile men. Total antioxidant capacity of the seminal plasma as measured by the luminоmetric assay is a reliable and simple test for diagnosing and management of male infertility.
Keywords
seminal plasma, male infertility, oxidative capacity